6533b83afe1ef96bd12a7189

RESEARCH PRODUCT

Acid excreting mutants of yeast Saccharomyces cerevisiae.

T.m. LachowiczEwa Boniewska-bernackaRenata GrochowalskaLucyna SłomińskaBeata Machnicka

subject

Saccharomyces cerevisiaeMutantCitric Acid CycleBiophysicsGlyoxylate cycleSaccharomyces cerevisiaeBiologyBiochemistrychemistry.chemical_compoundMolecular BiologyWaterCell BiologyHydrogen-Ion Concentrationbiology.organism_classificationYeastComplementationCitric acid cyclechemistryBiochemistryMutationbiology.proteinProtonsBromocresol purpleAcidsOxidation-ReductionOrganic anion

description

Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the excreted protons are associated to particular sets of organic anions such as citrate, aconitate, succinate, fumarate or malate. These novel types of respiratory mutations seem to be located in genes operating in the Krebs or glyoxylate cycle.

yearjournalcountryeditionlanguage
2004-10-19Biochemical and biophysical research communications
10.1016/j.bbrc.2004.10.130https://pubmed.ncbi.nlm.nih.gov/15541392