6533b83afe1ef96bd12a77ab
RESEARCH PRODUCT
Functional analysis of yeast gene families involved in metabolism of vitamins B1and B6
Bertrand LlorenteJosé E. Pérez-ortínMaría Teresa Rodríguez-manzanequeSusana Rodríguez-navarroBernard DujonDenis MarchesanAnna RamneGenoveva UberEnrique HerreroPer Sunnerhagensubject
GeneticsProtein familyFunctional analysisSaccharomyces cerevisiaeBioengineeringMetabolismBiologybiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistrychemistry.chemical_compoundBiochemistryBiosynthesischemistryGeneticsGene familyThiamineGeneBiotechnologydescription
In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1-3 and SNO1-3. In media lacking vitamin B(6), SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B(6) is essential for growth, copies 2 and 3 seem more related with B(1) biosynthesis during the exponential phase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-09-23 | Yeast |