6533b850fe1ef96bd12a8255

RESEARCH PRODUCT

Size characterisation of Au nanoparticles by ICP-MS coupling techniques

Jörg BettmerWolfram BrüchertAndreas Helfrich

subject

Gel electrophoresisReproducibilityDynamic light scatteringTransmission electron microscopyChemistryChemical structureAnalytical chemistryNanoparticleMass spectrometryInductively coupled plasma mass spectrometrySpectroscopyAnalytical Chemistry

description

In modern nanotechnology the properties of nanoparticles (NPs) in different application fields is mainly dependent on their size and their size distribution. This paper aims to develop a reliable method for the size characterisation of Au NPs. Basing on the combination of two different separation techniques (liquid chromatography and gel electrophoresis), coupled on-line to inductively coupled plasma-mass spectrometry (ICP-MS), we study the separation of Au NP standards. Separations by liquid chromatography show size dependent retention behaviour with good reproducibility (RSD < 1%). The results are compared with complementary methods like dynamic light scattering (DLS) and transmission electron microscopy (TEM) and show good agreement. Furthermore, the on-line coupling of gel electrophoresis and ICP-MS results in a distinct peak tailing for the analysis of these NPs, which is found to be related to the chemical structure of the NP’s surface. It can be demonstrated that NPs with a covalently bound surface do behave in a different way.

yearjournalcountryeditionlanguage
2006-01-01Journal of Analytical Atomic Spectrometry
https://doi.org/10.1039/b511705d