6533b851fe1ef96bd12a8c2b

RESEARCH PRODUCT

Non-isotopic DNA fingerprint analyses with the minisatellite probe MZ1.3

B. HoltkampE. BohnkeU. SchackerH.-h Sonneborn

subject

PhysicsMinisatelliteDNA profilingFingerprintNon isotopicResolution (electron density)Signal intensityRestriction fragment length polymorphismBiological system

description

RFLP analyses with minisatellite probes yield highly informative individual specific banding patterns (genetic fingerprints) (Jeffreys et al.). Because of the complexity of the pattern and marked differences in band intensities a good band resolution and high sensitivity of the probe are essential. By reason of the latter requirement to date most of the RFLP-studies are performed with radioactive probes. Although non-isotopic labels have been introduced into fingerprint analyses (Schafer et al.; Medeiros et al.) 32P-labeled probes are still superior with respect to sensitivity. Our approach to increase the specific signal intensity makes use of an amplification of the number of probe molecules bound per target molecule. The minisatellite probe MZ1.3 we describe here shows a 20-fold target-specific amplification in binding, facilitating the use of non-isotopic detection techniques.

yearjournalcountryeditionlanguage
1990-01-01
https://doi.org/10.1007/978-3-642-75496-8_40