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RESEARCH PRODUCT
Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene
Justina KavaliauskaitėAuksė KazlauskaitėJuozas Rimantas LazutkaGatis MozolevskisArūnas Stirkėsubject
QH301-705.5Microsecond pulsed electric fieldSecreted alkaline phosphataseReporter assaymicrosecond pulsed electric field; inducible gene transcription control; reporter assay; secreted alkaline phosphatase; mammalian cells; cell line; NF-κBTransfectionCatalysisArticleNF-κBInorganic Chemistry03 medical and health sciencesMice0302 clinical medicineElectricityinducible gene transcription controlAnimalsHumansmammalian cellsBiology (General)Physical and Theoretical ChemistryInducible gene transcription controlQD1-999Molecular BiologySpectroscopy030304 developmental biology0303 health sciencessecreted alkaline phosphataseOrganic ChemistryNF‐κBreporter assayNF-kappa BMammalian cells:NATURAL SCIENCES::Physics [Research Subject Categories]General Medicinecell linemicrosecond pulsed electric field3. Good healthComputer Science ApplicationsChemistryGene Expression Regulation030220 oncology & carcinogenesismicrosecond pulsed electric field ; inducible gene transcription control ; reporter assay ; secreted alkaline phosphatase ; mammalian cells ; cell line ; NF-κBCell linedescription
The possibility to artificially adjust and fine‐tune gene expression is one of the key mile-stones in bioengineering, synthetic biology, and advanced medicine. Since the effects of proteins or other transgene products depend on the dosage, controlled gene expression is required for any ap-plications, where even slight fluctuations of the transgene product impact its function or other critical cell parameters. In this context, physical techniques demonstrate optimistic perspectives, and pulsed electric field technology is a potential candidate for a noninvasive, biophysical gene regulator, exploiting an easily adjustable pulse generating device. We exposed mammalian cells, transfected with a NF‐κB pathway‐controlled transcription system, to a range of microsecond‐duration pulsed electric field parameters. To prevent toxicity, we used protocols that would generate relatively mild physical stimulation. The present study, for the first time, proves the principle that microsecond‐duration pulsed electric fields can alter single‐gene expression in plasmid context in mammalian cells without significant damage to cell integrity or viability. Gene expression might be upregulated or downregulated depending on the cell line and parameters applied. This noninvasive, ligand‐, cofactor‐, nanoparticle‐free approach enables easily controlled direct electrostimulation of the construct carrying the gene of interest; the discovery may contribute towards the path of simplification of the complexity of physical systems in gene regulation and create further synergies between electronics, synthetic biology, and medicine. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. --//-- Citation: Kavaliauskaite, J.; Kazlauskaite, A.; Lazutka, J.R.; Mozolevskis, G.; Stirke, A. Pulsed Electric Fields Alter Expression of NF-κB Promoter Controlled Gene. Int. J. Mol. Sci. 2022, 23, 451. https://doi.org/10.3390/ijms23010451. Article published under the CC BY 4.0 license.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-12-31 | International Journal of Molecular Sciences |