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RESEARCH PRODUCT

Fast UPLC/PDA determination of squalene in Sicilian P.D.O. pistachio from Bronte: Optimization of oil extraction method and analytical characterization

Vita Di StefanoValentina Giusi CapocchianoEmanuele SaijaGiacomo DugoAndrea SalvoGiovanna Loredana La TorreVito PellizzeriKatia Erminia CasaleValentina Mangano

subject

0301 basic medicineSqualeneResolution (mass spectrometry)Settore CHIM/10 - Chimica Degli AlimentiUplc pda01 natural sciencesHigh-performance liquid chromatographyAnalytical Chemistry03 medical and health sciencesSqualenechemistry.chemical_compoundLimit of DetectionNutsPlant OilsSicilySqualene; pistachio (Pistacia vera L.); Food analysis; Green pistachio Bronte (P.D.O.); UPLC/PDA analysis030109 nutrition & dieteticsChromatographyChemistryPlant Extracts010401 analytical chemistryExtraction (chemistry)Reproducibility of ResultsGeneral MedicineSqualene pistachio (Pistacia vera L.) food analysis Green Pistachio Bronte (P.D.O.) UPLC/PDA analysis.Squalene pistachio (Pistacia vera L.) Food analysis Green pistachio Bronte (P.D.O.) UPLC/PDA analysis0104 chemical sciencesPistaciaExtraction methodsFood AnalysisFood Science

description

Abstract A fast reversed-phase UPLC method was developed for squalene determination in Sicilian pistachio samples that entry in the European register of the products with P.D.O. In the present study the SPE procedure was optimized for the squalene extraction prior to the UPLC/PDA analysis. The precision of the full analytical procedure was satisfactory and the mean recoveries were 92.8 ± 0.3% and 96.6 ± 0.1% for 25 and 50 mg L−1 level of addition, respectively. Selected chromatographic conditions allowed a very fast squalene determination; in fact it was well separated in ∼0.54 min with good resolution. Squalene was detected in all the pistachio samples analyzed and the levels ranged from 55.45–226.34 mg kg−1. Comparing our results with those of other studies it emerges that squalene contents in P.D.O. Sicilian pistachio samples, generally, were higher than those measured for other samples of different geographic origins.

yearjournalcountryeditionlanguage
2017-04-01
10.1016/j.foodchem.2016.10.126http://hdl.handle.net/11570/3096852