6533b851fe1ef96bd12a8e48

RESEARCH PRODUCT

Additional file 9: of SWATH-MS based quantitative proteomics analysis reveals that curcumin alters the metabolic enzyme profile of CML cells by affecting the activity of miR-22/IPO7/HIF-1α axis

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Figure S4. Effects of Curcumin on HIF-1α activity, IPO7 expression and miR22 expression in LAMA84 cells. a Assay of the transcriptional activity of HIF-1α showing that in LAMA84 cells curcumin induced a reduction of HIF-1α activity compared to control cells. The reported values are the mean of three independent experiments. b qPCR (left panel) and representative Western blot (right panel) show that in LAMA84 cells curcumin treatment did not affect HIF-1α at both mRNA and protein level. The values (FOI: Fold of Induction) in the histogram are normalized against GAPDH and are the mean ± SD of three independent experiments. c qPCR demonstrates that in LAMA84 cells curcumin induced a decrease of mRNA IPO7 expression. The values (FOI: Fold of Induction) in the histogram are normalized to GAPDH and are the mean ± SD of three independent experiments. d Representative western blot and corresponding densitogram showing that in LAMA84 cells curcumin inhibited the protein expression of IPO7. e qRT-PCR showing the ability of curcumin to induce in LAMA84 cells a significant increase of miR-22 expression. The values (FOI: Fold of Induction) in the histogram are normalized against RNU6–2 and are the mean ± SD of two independent experiments. In the Western blot assay, actin was used as loading control. Intensities of proteins bands were calculated from the peak area of densitogram by using Image J software. Ctrl: control cells. Statistical significance was calculated vs Ctrl: *p