6533b851fe1ef96bd12a986f
RESEARCH PRODUCT
Impaired cellular cholesterol efflux by oxysterol-enriched high density lipoproteins.
Denis BlacheNadine LoreauLaurence Gesquièresubject
Chromatography GasOxysterolBiochemistryThiobarbituric Acid Reactive SubstancesCell LineExcretionchemistry.chemical_compoundMicePhysiology (medical)MalondialdehydeCellular cholesterolAnimalsHumansKetocholesterolsCholesterolMacrophagesReverse cholesterol transportMalondialdehydeHydroxycholesterolsCholesterolchemistryBiochemistryCell culturelipids (amino acids peptides and proteins)EffluxLipoproteins HDLOxidation-ReductionCopperdescription
One of the proposed antiatherogenicity role of high-density lipoproteins (HDL) is believed to stimulate removal of cholesterol from the peripheral cells back to the liver for excretion. We have investigated the effects of oxidation-related modifications of HDL on their ability to stimulate cholesterol efflux from cultured cells. Human HDL (HDL3, 1.13 < d < 1.21 g/ml) have been modified either by malondialdehyde or by copper-mediated oxidation (Ox-HDL3). Compared with native HDL3, the modified HDL3 resulted in a significantly reduced efflux of labeled cholesterol from preloaded macrophages (P388D1 cell line). Analysis of lipid composition of Ox-HDL3 by gas chromatography revealed the presence of oxysterols (OS). Enrichment of native HDL3 with oxysterols resulted in a reduced capacity to stimulate cholesterol efflux. The reduced ability of OS-enriched HDL3 to elicit cholesterol efflux may contribute to cellular cholesterol accumulation and subsequently to atherosclerosis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-01-01 | Free radical biologymedicine |