6533b851fe1ef96bd12a997a
RESEARCH PRODUCT
Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.
M. Luz CandenasElsa AnselmiJosefina MagranerPilar AusinaFrancisco M. PintoJulio D. Martínsubject
medicine.medical_specialtyanimal structuresNk3 receptorDNA Complementarymedicine.drug_classOvariectomyUterusBiologydigestive systemcomplex mixturesPolymerase Chain ReactionInternal medicineGene expressionmedicineAnimalsRNA MessengerRats WistarProgesteronePharmacologyElectrophoresis Agar GelMessenger RNAurogenital systemmusculoskeletal neural and ocular physiologyUterusEstrogensReceptors Neurokinin-3RatsEndocrinologymedicine.anatomical_structureGene Expression RegulationEstrogenIn uteroRat uterusOvariectomized ratFemaledescription
The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not progesterone, regulates the expression of tachykinin NK3 receptors in the rat uterus.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-04-01 | European journal of pharmacology |