6533b851fe1ef96bd12a9a00

RESEARCH PRODUCT

The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature

Paola SalernoSandra MarineoAnna Maria Puglia

subject

OperonMolecular Sequence Datagenetic processesRegulatorStreptomyces coelicolorBiochemistryMicrobiologyheat shock responseBacterial ProteinsTranscription (biology)Heat shock proteinOperonGeneticsHSP70 Heat-Shock ProteinsHeat shockPromoter Regions GeneticMolecular BiologyGeneHeat-Shock ProteinsGeneticsBase SequencebiologyReverse Transcriptase Polymerase Chain ReactionStreptomyces coelicolorTemperatureGene Expression Regulation BacterialGeneral Medicinebiology.organism_classificationRepressor ProteinshspRChaperone (protein)biological sciencesbiology.proteinbacteria

description

HspR (heat shock protein regulator) acts as a negative regulator of different genes in many bacteria. In Streptomyces coelicolor hspR gene is part and the transcriptional repressor of the dnaK operon which encodes the DnaK, GrpE, DnaJ chaperone machines and HspR itself. Our experiments led us to the discovery of a second promoter, internal to dnaK operon, located upstream hspR gene. Transcription from this promoter was detected at 30 degrees C indicating that hspR could play a key physiological role.

yearjournalcountryeditionlanguage
2006-09-04Archives of Microbiology
https://doi.org/10.1007/s00203-007-0269-y