6533b851fe1ef96bd12a9a56
RESEARCH PRODUCT
On-Line Derivatization into Precolumns for the Determination of Drugs by Liquid Chromatography and Column Switching: Determination of Amphetamines in Urine
Adela Sevillano-cabezaPilar Campí Ns-falcóRosa Herráez-hernándezsubject
AnalyteChromatographyAmphetaminesUrineChloroformateMethamphetamineAnalytical ChemistrySubstance Abuse Detectionchemistry.chemical_compoundColumn chromatographychemistryReagentIndicators and ReagentsColumn switchingDerivatizationQuantitative analysis (chemistry)Chromatography High Pressure Liquiddescription
A chromatographic system for the on-line derivatization of drugs using column switching is described. The system uses a 20 mm × 2.1 mm i.d. precolumn packed with a unmodified ODS stationary phase. This column is used for sample cleanup and enrichment of the analytes. Next, the trapped analytes are derivatized by injection of the derivatization reagent into the precolumn. Finally, the derivatives are transferred to the analytical column for their separation under reversed-phase conditions. The influence of several parameters such as the reaction time, the amount of derivatization reagent, or the system design has been studied using some amphetamines as model compounds and three derivatization reagents: sodium 1,2-naphthoquinone-4-sulfonate, o-phthaldialdehyde, and 9-fluorenylmethyl chloroformate. The potential of the described approach is illustrated by determining amphetamine and methamphetamine in untreated urine at ambient temperature.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-03-01 | Analytical Chemistry |