Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues

6533b852fe1ef96bd12aa31b

RESEARCH PRODUCT

Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues

Varpu Marjomäki Anni Honkimaa Mira Laajala Heikki Hyöty Kerttu Kalander Sami Oikarinen Sami Salmikangas Iryna Shanina Jutta E. Laiho Marc S. Horwitz

subject

0301 basic medicine Microbiology (medical)replication 030106 microbiology Cell In situ hybridization Biology branched DNA medicine.disease_cause infektiot Microbiology Article antiviral drugs 03 medical and health sciences chemistry.chemical_compound positive RNA Virology medicine BDNA test lcsh:QH301-705.5 replikaatio enterovirus virus diseases RNA Translation (biology)negative RNA Virology enterovirukset 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General)chemistry Virion assembly RNA Enterovirus in situ hybridization 3111 Biomedicine DNA

description

The current methods to study the distribution and dynamics of viral RNA molecules inside infected cells are not ideal, as electron microscopy and immunohistochemistry can only detect mature virions, and quantitative real-time PCR does not reveal localized distribution of RNAs. We demonstrated here the branched DNA in situ hybridization (bDNA ISH) technology to study both the amount and location of the emerging &minus

year	journal	country	edition	language
2020-12-01	Microorganisms

https://doi.org/10.3390/microorganisms8121928

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