6533b852fe1ef96bd12aa439
RESEARCH PRODUCT
Stable expression in HEK-293 cells of the rat alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor.
Ullrich EbbinghausUllrich EbbinghausAlfred MaelickeChristoph MethfesselAndré SchrattenholzGert KramerAlexander StorchLivia PoteurEva Stetzersubject
Stable expressionPatch-Clamp Techniquesα3/β4 nAChRBiophysicsNicotinic AntagonistsPharmacologyReceptors NicotinicTransfectionBiochemistryCell LineGanglionic nAChRCa2+ imagingGanglion type nicotinic receptorStructural BiologyMuscarinic acetylcholine receptorGeneticsmedicineAnimalsHumansNicotinic AgonistsNicotinic AntagonistHEK cellMolecular BiologyNeuronsurogenital systemChemistryMuscarinic acetylcholine receptor M3Cell BiologyAcetylcholineRecombinant ProteinsRatsNicotinic acetylcholine receptorNicotinic agonistCalciumCalcium ChannelsAlpha-4 beta-2 nicotinic receptorAcetylcholinemedicine.drugdescription
The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by single-cell and whole-cell patch-clamp studies. Acetylcholine (ACh) induced fast activating currents which showed desensitisation and inward rectification. The conductance of the ACh-activated channel was 29 pS. The order of potency of the nicotinic agonists tested was cytisine approximately = nicotine > acetylcholine. The EC50 value for ACh was 145 microM; the Hill coefficient was close to 2. The currents elicited by ACh were effectively blocked by nicotinic antagonists, but not by the muscarinic antagonist atropine. These properties are comparable to the pharmacological and physiological profile of ganglionic nicotinic receptors and type III currents of cultured hippocampal neurons.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-11-11 | FEBS letters |