6533b852fe1ef96bd12aa43d

RESEARCH PRODUCT

Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy

subject

description

Spatial light modulator (SLM) implementation of wavefront encoding enables various types of engineered pointspread functions (PSFs), including the generalized-cubic and squared-cubic phase mask wavefront encoded (WFE) PSFs, shown to reduce the impact of sample-induced spherical aberration in fluorescence microscopy. This investigation validates dynamic experimental parameter variation of these WFE-PSFs. We find that particular design parameter bounds exist, within which the divergence of computed and experimental WFE-PSFs is of the same order of magnitude as that of computed and experimental conventional PSFs, such that model-based approaches for solving the inverse imaging problem can be applied to a wide range of SLM-WFE systems. Interferometric measurements were obtained to evaluate the SLM implementation of the numeric mask. Agreement between experiment and theory in terms of a wrapped phase, 0–2π, validates the phase mask implementation and allows characterization of the SLM response. These measurements substantiate experimental practice of computational-optical microscope imaging with an SLM-engineered PSF. © 2015 Optical Society of America OCIS codes: (110.0110) Imaging systems; (180.0180) Microscopy; (230.6120) Spatial light modulators; (110.7348) Wavefront