6533b852fe1ef96bd12aa492

RESEARCH PRODUCT

Fatty Acid Transporter CD36 Mediates Hypothalamic Effect of Fatty Acids on Food Intake in Rats

Christophe GuissardValentine S. MoulléJulien DairouChristophe MagnanAnne LorsignolBarry E. LevinClaude RouchLinh-chi BuiErwann PhilippeCéline Cruciani-guglielmacciChristelle Le FollLuc PénicaudNadim KassisNicolas Marsollier

subject

CD36 AntigensMaleMicrodialysismedicine.medical_specialty[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyCD36HypothalamusGene Expressionlcsh:MedicineModels BiologicalEating03 medical and health scienceschemistry.chemical_compound0302 clinical medicineIn vivoInternal medicinemedicineAnimalslcsh:SciencePhospholipids030304 developmental biology2. Zero hungerchemistry.chemical_classification0303 health sciencesMultidisciplinaryTriglyceridebiologyFatty Acidslcsh:RNeurosciences[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyFatty acidFeeding BehaviorFatty Acid Transport ProteinsRatsSoybean OilTriacsin CEndocrinologychemistryHypothalamus[ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyNeurons and Cognitionbiology.proteinEmulsionslcsh:QProto-Oncogene Proteins c-fos030217 neurology & neurosurgeryEtomoxirResearch Article

description

Subject Areas: carotid arteries; emulsions; fatty acids; gene expression; heparin; hypothalamus; neurons; oxidation.; International audience; Variations in plasma fatty acid (FA) concentrations are detected by FA sensing neurons in specific brain areas such as the hypothalamus. These neurons play a physiological role in the control of food intake and the regulation of hepatic glucose production. Le Foll et al. previously showed in vitro that at least 50% of the FA sensing in ventromedial hypothalamic (VMH) neurons is attributable to the interaction of long chain FA with FA translocase/CD36 (CD36). The present work assessed whether in vivo effects of hypothalamic FA sensing might be partly mediated by CD36 or intracellular events such as acylCoA synthesis or β-oxidation. To that end, a catheter was implanted in the carotid artery toward the brain in male Wistar rats. After 1 wk recovery, animals were food-deprived for 5 h, then 10 min infusions of triglyceride emulsion, Intralipid +/- heparin (IL, ILH, respectively) or saline/heparin (SH) were carried out and food intake was assessed over the next 5 h. Experimental groups included: 1) Rats previously injected in ventromedian nucleus (VMN) with shRNA against CD36 or scrambled RNA; 2) Etomoxir (CPT1 inhibitor) or saline co-infused with ILH/SH; and 3) Triacsin C (acylCoA synthase inhibitor) or saline co-infused with ILH/SH. ILH significantly lowered food intake during refeeding compared to SH (p<0.001). Five hours after refeeding, etomoxir did not affect this inhibitory effect of ILH on food intake while VMN CD36 depletion totally prevented it. Triacsin C also prevented ILH effects on food intake. In conclusion, the effect of FA to inhibit food intake is dependent on VMN CD36 and acylCoA synthesis but does not required FA oxidation.

https://doi.org/10.1371/journal.pone.0074021