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RESEARCH PRODUCT

Determination of Phenols from Automobile Exhaust by Means of High-Performance Liquid Chromatography (HPLC)

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Abstract The paper describes a procedure to determine ten representative phenols (phenol, m-, p-, o-cresol, 2,4-, 2,6-, 3,4-, 3,5-xylenol, 2,3,6-, 2,4,6-trimethylphenol) in engine exhaust. Sampling was performed by a conventional Grimmer device. A sample of the water condensate collected was extracted with ether. After concentration of the ether extract phenols were isolated from other interfering compounds by stepwise elution on a Lobar column packed with LiChroprep Si 60 under controlled conditions. Relative recovery of the majority of reference phenols ranged from 90-100%. Separation of phenols into individual components was performed on polar packings as well as on Reverse Phase packings. Quantitative analysis was carried out on a prepacked Zorbax ODS column of 250 × 4.6 mm in acetonitrile/water of 30,170 (v/v) using a UV photometer of 254 nm wavelength. Phenols in the extract of the exhaust were identified on the basis of retention times and by spectroscopic means. Quantitative analysis was performed...