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RESEARCH PRODUCT
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Stefan HaakKristin MetzdorfMartin HeineMartin HeineSteffen FrickeMartin KorteMelanie OhmMarta Zagrebelskysubject
0301 basic medicineHippocampal formationInhibitory postsynaptic potentialGeneral Biochemistry Genetics and Molecular BiologyArticleSynaptic plasticityDephosphorylation03 medical and health sciences0302 clinical medicineSingle Particle Trackingmental disordersEi BalanceVeröffentlichung der TU Braunschweiglcsh:QH301-705.5Loss functionExcitationS1pr2S1PR2ddc:5InhibitionChemistryQuantum dotsCalcineurinGabaarsNogo-A; S1PR2 ; EI balance ; calcineurin ; inhibition ; excitation ; quantum dots ; GABAARs ; synaptic plasticity ; single particle trackingddc:57030104 developmental biologylcsh:Biology (General)Synaptic plasticityExcitatory postsynaptic potentialGABAergicNogo-ANeurosciencepsychological phenomena and processes030217 neurology & neurosurgerydescription
Summary: Precisely controlling the excitatory and inhibitory balance is crucial for the stability and information-processing ability of neuronal networks. However, the molecular mechanisms maintaining this balance during ongoing sensory experiences are largely unclear. We show that Nogo-A signaling reciprocally regulates excitatory and inhibitory transmission. Loss of function for Nogo-A signaling through S1PR2 rapidly increases GABAAR diffusion, thereby decreasing their number at synaptic sites and the amplitude of GABAergic mIPSCs at CA3 hippocampal neurons. This increase in GABAAR diffusion rate is correlated with an increase in Ca2+ influx and requires the calcineurin-mediated dephosphorylation of the γ2 subunit at serine 327. These results suggest that Nogo-A signaling rapidly strengthens inhibitory GABAergic transmission by restricting the diffusion dynamics of GABAARs. Together with the observation that Nogo-A signaling regulates excitatory transmission in an opposite manner, these results suggest a crucial role for Nogo-A signaling in modulating the excitation and inhibition balance to restrict synaptic plasticity. : Fricke et al. explore the molecular mechanisms regulating the strength of inhibitory synaptic transmission and the GABAAR localization at inhibitory synapses. Nogo-A/S1PR2 signaling rapidly strengthens inhibitory synaptic transmission in the hippocampus, limits GABAAR diffusion dynamics, and promotes GABAAR clustering at synapses in a Ca2+- and calcineurin-dependent manner. Keywords: EI balance, inhibition, excitation, synaptic plasticity, GABAARs, quantum dots, single particle tracking, Nogo-A, S1PR2, calcineurin
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-10-01 |