6533b852fe1ef96bd12ab74e
RESEARCH PRODUCT
Antibiotic resistance and population structure of cystic fibrosis Pseudomonas aeruginosa isolates from a Spanish multi-centre study
Carla López-causapéJuan De Dios-caballeroMarta CoboAmparo EscribanoÓScar AsensioAntonio OliverRosa Del CampoRafael CantónAmparó SoléIsidoro CortellOscar AsensioGloria GarcíaMaría Teresa MartínezMaría ColsAntonio SalcedoCarlos VázquezFélix BarandaRosa GirónEsther QuintanaIsabel DelgadoMaría ÁNgeles De MiguelMarta GarcíaConcepción OlivaMaría Concepción PradosMaría Isabel BarrioMaría Dolores PastorCasilda OlveiraJavier De GraciaAntonio ÁLvarezAmparo EscribanoSilvia CastilloJoan FiguerolaBernat TogoresAntonio OliverCarla LópezJuan De Dios CaballeroMarta TatoLuis MáizLucrecia SuárezRafael Cantónsubject
0301 basic medicineMaleCystic FibrosisAntibiotic resistanceArray tubeMulti-locus sequence typing (MLST)medicine.disease_causeGenotypePharmacology (medical)ChildGeneticseducation.field_of_studyMolecular EpidemiologyVirulencePseucforrumus aeruginosaGeneral MedicineMiddle AgedMutS DNA Mismatch-Binding ProteinElectrophoresis Gel Pulsed-FieldInfectious DiseasesChild PreschoolPseudomonas aeruginosaFemalemedicine.drugMicrobiology (medical)AdultAdolescentGenotype030106 microbiologyPopulationVirulenceBiologyCystic fibrosisMicrobiology03 medical and health sciencesYoung AdultAntibiotic resistanceDrug Resistance BacterialmedicineHumansPseudomonas InfectionsTypingeducationGenetic diversityPseudomonas aeruginosaGenetic VariationMutL ProteinsSpainColistinMultilocus Sequence Typingdescription
The first Spanish multi-centre study on the microbiology of cystic fibrosis (CF) was conducted from 2013 to 2014. The study involved 24 CF units from 17 hospitals, and recruited 341 patients. The aim of this study was to characterise Pseudomonas aeruginosa isolates, 79 of which were recovered from 75 (22%) patients. The study determined the population structure, antibiotic susceptibility profile and genetic background of the strains. Fifty-five percent of the isolates were multi-drug-resistant, and 16% were extensively drug-resistant. Defective mutS and mutL genes were observed in mutator isolates (15.2%). Considerable genetic diversity was observed by pulsed-field gel electrophoresis (70 patterns) and multi-locus sequence typing (72 sequence types). International epidemic clones were not detected. Fifty-one new and 14 previously described array tube (AT) genotypes were detected by AT technology. This study found a genetically unrelated and highly diverse CF P. aeruginosa population in Spain, not represented by the epidemic clones widely distributed across Europe, with multiple combinations of virulence factors and high antimicrobial resistance rates (except for colistin). (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-01 |