6533b853fe1ef96bd12abf78

RESEARCH PRODUCT

New Tools for Streamlined In Vivo Homing Peptide Identification

Karlis PleikoKarlis PleikoTambet TeesaluTambet TeesaluTambet TeesaluMaarja HaugasKristina Põšnograjeva

subject

chemistry.chemical_classificationComputingMethodologies_PATTERNRECOGNITIONT7 bacteriophagechemistryIn vivoComputer sciencePeptideIdentification (biology)BiopanningComputational biologyHoming (hematopoietic)

description

In vivo peptide-phage display is an unbiased technique for mapping of the vascular diversity and identification of homing peptides. This chapter is intended to serve as a structured practical guide to execute in vivo T7 phage biopanning and data analysis experiments. We discuss experimental designs and protocols with emphasis on application of high-throughput sequencing-based technologies for streamlined in vivo biopanning and validation of homing peptides.

yearjournalcountryeditionlanguage
2021-11-12
https://doi.org/10.1007/978-1-0716-1752-6_25