6533b853fe1ef96bd12ac249
RESEARCH PRODUCT
Resistance of a recombinant Escherichia coli to dehydration.
Galina KhroustalyovaAlexander RapoportJoseph Boudrantsubject
0106 biological sciencesMicroorganismPreservation BiologicalBiologymedicine.disease_cause01 natural scienceslaw.inventionRecombinant strain03 medical and health scienceschemistry.chemical_compoundlaw010608 biotechnologymedicineEscherichia coli[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringDehydrationDesiccationCryptobiosisIncubationEscherichia coli030304 developmental biologyRecombination Genetic0303 health sciencesMicrobial ViabilityDehydrationTrehaloseCell BiologyGeneral MedicineRehydrationmedicine.diseaseAnhydrobiosisTrehaloseCell resistanceDistilled waterBiochemistrychemistryRecombinant DNAdescription
International audience; Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a 88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-01-01 | Cell biology international |