6533b853fe1ef96bd12ac27a
RESEARCH PRODUCT
Mapping antigenic epitopes of potato virus Y with antibodies affinity-purified by using overlapping synthetic peptides
Maija Vihinen-rantaReijo SironenMatti Vuentosubject
chemistry.chemical_classificationbiologylcsh:SPeptidekasviviruksetbiology.organism_classificationSolanum tuberosumlcsh:S1-972VirologyPVYEpitopelcsh:AgriculturePepscanPotato virus YchemistryAntigencoat proteinbiology.proteinlcsh:Agriculture (General)AntibodyFood Sciencedescription
Synthetic, overlapping peptides representing the entire amino acid sequence of potato virus Y (PVY) coat protein were used to affinity-purify antibodies from polyclonal antisera to PVY. In testing the binding of the purified antibodies to PVY particles, antigenic epitopes were identified. The N-terminal and C-terminal regions of the PVY coat protein were found to contain most of the antigenic epitopes. The results will facilitate the development of detection methods for PVY based on synthetic peptides.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-03-01 | Agricultural and Food Science |