6533b853fe1ef96bd12ac2ab
RESEARCH PRODUCT
Molar growth yields ofZymomonas mobilis on glucose after the transition from anaerobic to aerobic continuous growth
L. AuzinaPeteris ZikmanisR. Krucesubject
biologyAcetaldehydeBiomassBioengineeringbiology.organism_classificationApplied Microbiology and BiotechnologyZymomonas mobilisDilutionchemistry.chemical_compoundBiochemistrychemistryYield (chemistry)Steady state (chemistry)Anaerobic exerciseBacteriaBiotechnologyNuclear chemistrydescription
An increase in the molar growth yield (Y X/S = 14.3-20.3 g/mol) on glucose (25 mM) was achieved after the transition of Zymomonas mobilis ATCC 29191 from anaerobic to aerobic steady state growth at dilution rates of D = 0.31-0.40 1/h and under oxygen-unlimited conditions. The transfer of anaerobically or aerobically grown steady state cells into a fresh medium resulted in the higher values of Y X/S . A positive correlation was established between biomass and acetaldehyde yield within the range of 5-9 mM acetaldehyde in the medium. An inhibitory effect of the exogenously added acetaldehyde (Kj = 16.7 ± 2.8 mM) on the ATPase activity was observed in vitro, using cell-free extracts of anaerobically grown Z mobilis. The results obtained provide evidence that the increased values of biomass yield could be explained by the redirection of ATP usage during aerobic growth of Z mobilis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 | Acta Biotechnologica |