6533b853fe1ef96bd12aca57

RESEARCH PRODUCT

Cloning of sponge heat shock proteins: evolutionary relationships between the major kingdoms

subject

description

In the present study we have cloned from sponges (Porifera) those molecules which are involved in the protection of organisms against physiological and stress conditions; the inducible heat shock protein Mr 70,000, hsp70, from the marine sponge Geodia cydonium, its interacting hsp40, a DnaJ-like protein (from G. cydonium) and the constitutively expressed counterpart the glucose-regulated protein Mr 78,000, GRP78 from Suberites domuncula. Alignments of the sequences revealed that the deduced aa sequences of all sponge hsp's share high homology to other metazoan sequences, and are separated from related sequences from plants and fungi (hsp70, GRP78, DnaJ) as well as Bacteria (DnaK, the hsp70 homologoue and the DnaJ) and Archaea (DnaK, the hsp70 homologoue and the DnaJ). One comparison based on nt sequences (hsp70/DnaK) showed a less pronounced grouping. From these data we conclude, that for phylogenetic analyses of deep branches in the metazoan evolution, not only ‘characteristic’metazoan genes, but also ‘housekeeping genes’e.g. are suitable for evolutionary inference. The sequences reported here have been submitted to the EMBL/GenBank data base (accession no. GCDNAJ Y09037, GCHSP70 X94985, SDGR78 Y09500).