6533b853fe1ef96bd12ace17

RESEARCH PRODUCT

Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa

Antonio PellicerAntonio PellicerA RuizJosé RemohíJosé RemohíM Gil-salomC. RubioJ.l.l. Romero

subject

AdultMaleendocrine systemAdolescentPregnancy RateCleavage Stage Ovummedicine.medical_treatmentBiochemistryCryopreservationIntracytoplasmic sperm injectionMale infertilityAndrologyEndocrinologyHuman fertilizationPregnancyTestisHumansMedicineSperm Injections IntracytoplasmicMolecular Biologyreproductive and urinary physiologyRetrospective StudiesCryopreservationAzoospermiaurogenital systembusiness.industrymedicine.diseaseSpermatozoaSpermTesticular sperm extractionPregnancy rateFertilizationFemalebusinessSemen Preservation

description

To assess if testicular sperm cryopreservation is a valid alternative to repetition of testicular sperm retrieval techniques, results of a cryopreservation technique in pills have been retrospectively analyzed. Enough motile spermatozoa for ICSI were obtained in 172 from 190 (90.5%) frozen-thawed testicular sperm samples. Overall, 249 couples underwent 390 ICSI cycles, 156 using fresh and 234 using cryopreserved testicular sperm. Mean two-pronuclear fertilization rates per cycle were not significantly different after ICSI with fresh (62.0%) or with cryopreserved (63.2%) spermatozoa. Mean embryo cleavage rate per cycle was higher in the fresh (90.6%) than in the cryopreserved (84.6%) group (P = 0.016). However, clinical pregnancy rates per cycle (28.2% with fresh vs 27.8% with cryopreserved), implantation rates (12.2% vs 13.1%) and ongoing pregnancy rates per cycle (22.4% vs 21.8%) were not significantly different. Cryopreservation of testicular spermatozoa is an effective technique that can be used both in obstructive and in non-obstructive azoospermia.

yearjournalcountryeditionlanguage
2001-01-13Molecular and Cellular Endocrinology
https://doi.org/10.1016/s0303-7207(00)00345-2