6533b853fe1ef96bd12ad542
RESEARCH PRODUCT
A synthetic derivative of antimicrobial peptide holothuroidin 2 from mediterranean sea cucumber (Holothuria tubulosa) in the control of Listeria monocytogenes
Maria Grazia CusimanoMaria VitaleAngelo SpinelloVincenzo ArizzaBarbara ParrinoStella CascioferroAlessandra MagistratoDomenico SchillaciGiampaolo Baronesubject
antimicrobial peptideAntibioticsSettore BIO/05 - ZoologiaPharmaceutical SciencePeptideSettore BIO/19 - Microbiologia Generalemedicine.disease_cause01 natural sciencesFoodborne DiseasesDrug DiscoveryListeriosislcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)chemistry.chemical_classification0303 health sciencesbiologyBiofilmFoodborne pathogenAntimicrobialHolothuria tubulosaAnti-Bacterial AgentsSettore CHIM/03 - Chimica Generale E InorganicaAntimicrobial peptidesmedicine.drug_classAntimicrobial peptides-Microbial Sensitivity TestsMolecular Dynamics SimulationArticleMicrobiology03 medical and health sciencesListeria monocytogenesDrug Resistance BacterialmedicineMediterranean SeaAnimalsHolothuria<i>Holothuria tubulosa</i>Listeria monocytogene030304 developmental biology010405 organic chemistryHolothuria tubulosaBiofilmbiology.organism_classificationSettore CHIM/08 - Chimica FarmaceuticaListeria monocytogenesProtein tertiary structure0104 chemical sciencesProtein Structure TertiaryFoodborne pathogenslcsh:Biology (General)chemistryBiofilmsDrug Design<i>Listeria monocytogenes</i>Antimicrobial Cationic Peptidesdescription
Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-03-08 |