6533b853fe1ef96bd12ad579

RESEARCH PRODUCT

Cytotoxicity, Genotoxicity and Disturbance of Cell Cycle in HepG2 Cells Exposed to OTA and BEA: Single and Combined Actions

Cristina JuanAna Juan-garcíaJosefa TolosaMaría-josé Ruiz

subject

Ochratoxin AFusariumCell SurvivalHealth Toxicology and Mutagenesislcsh:MedicineToxicologymedicine.disease_causeArticle03 medical and health scienceschemistry.chemical_compound0404 agricultural biotechnologyDepsipeptidesmedicineHumansDrug InteractionsMTT assayFood scienceMycotoxinHepG2 cells030304 developmental biology0303 health sciencesMicronucleus Testsbiologybeauvericingenotoxicitylcsh:Rfood and beveragesHep G2 Cells04 agricultural and veterinary sciencesbiology.organism_classificationOchratoxins040401 food scienceBeauvericinmixtureschemistryPenicilliumcell cycleMicronucleusochratoxin AGenotoxicityDNA Damage

description

Mycotoxins are produced by a number of fungal genera spp., for example, Aspergillus, Penicillium, Alternaria, Fusarium, and Claviceps. Beauvericin (BEA) and Ochratoxin A (OTA) are present in various cereal crops and processed grains. This goal of this study was to determine their combination effect in HepG2 cells, presented for the first time. In this study, the type of interaction among BEA and OTA through an isobologram method, cell cycle disturbance by flow cytometry, and genotoxic potential by in vitro micronucleus (MN) assay following the TG 487 (OECD, 2016) of BEA and OTA individually and combined in HepG2 cells are presented. Cytotoxic concentration ranges studied by the MTT assay over 24, 48, and 72 h were from 0 to 25 &micro

yearjournalcountryeditionlanguage
2019-06-01Toxins
10.3390/toxins11060341https://www.mdpi.com/2072-6651/11/6/341