6533b854fe1ef96bd12adda4

RESEARCH PRODUCT

Parallel Measurements of in-vivo Skin Autofluorescence Lifetimes and Photobleaching Rates

Jānis SpigulisAlexey LihachevD. ChorvatInesa Ferulova

subject

AutofluorescenceFluorescence intensityMaterials scienceOpticsPulse (signal processing)In vivobusiness.industrySkin autofluorescencebusinessPhotobleachingPhoton countingExcitation

description

Experimental methodology for parallel measurements of in-vivo skin autofluorescence (AF) lifetimes and photo-bleaching dynamic has been developed and tested. The AF lifetime decay distributions were periodically collected from fixed tissue area with subsequent detection of the fluorescence intensity decrease dynamic at different time shifts after the pulse excitation. Temporal distributions of skin AF lifetimes and bleaching dynamic were collected and analyzed by means of commercial time-correlated single photon counting system. Details of the equipment and data processing are described as well as some measurement results that confirm the feasibility of the proposed technology.

yearjournalcountryeditionlanguage
2015-01-01
https://doi.org/10.1007/978-3-319-12967-9_21