6533b854fe1ef96bd12ae97d

RESEARCH PRODUCT

Optimization of electrospraying conditions for the microencapsulation of probiotics and evaluation of their resistance during storage and in-vitro digestion

Rocío Pérez-masiáRussell Cruz MorfinLaura G. Gómez-mascaraqueGloria SánchezGloria SánchezAmparo López-rubio

subject

0301 basic medicineWhey proteinmedicine.medical_treatmentMicroorganismProbioticlaw.invention03 medical and health sciencesProbiotic0404 agricultural biotechnologylawmedicineViability assayFood scienceElectrospraying030109 nutrition & dieteticsbiologyChemistryPrebiotic04 agricultural and veterinary sciencesIn vitro digestionbiology.organism_classification040401 food scienceWhey proteinSurface response methodologyL. plantarumEncapsulationLactobacillus plantarumFood Science

description

Electrospraying has recently emerged as a novel microencapsulation technique with potential for the protection of probiotics. However, research efforts are still needed to minimize the viability loss observed during the processing of sensitive strains, and to maximize productivity. The aim of the present work was the optimization of the electrospraying conditions for the microencapsulation of a model probiotic microorganism, Lactobacillus plantarum, within a whey protein concentrate matrix. In a pre-optimization step, the convenience of encapsulating fresh culture instead of freeze-dried bacteria was established. Additionally, a surface response methodology was used to study the effect of the applied voltage, surfactant concentration, and addition of a prebiotic to the formulation on cell viability and productivity. Viability losses lower than 1 log10 CFU were achieved and the bacterial counts of the final products exceeded 8.5 log10 CFU/g. The protection ability of the developed structures during storage and in-vitro digestion was also evaluated.

10.1016/j.lwt.2016.01.071http://hdl.handle.net/10261/132963