6533b854fe1ef96bd12aeb86

RESEARCH PRODUCT

p-Chlorophenylalanine treatment depresses the number of synaptic ribbon profiles in the rat pineal gland, but does not abolish their day-night rhythm

subject

description

It is largely unknown as to how the day/night rhythm of pineal synaptic ribbon number is regulated. Transcriptional events related to the nocturnal formation of new synaptic ribbons occur early in the morning, when pineal serotonin levels begin to increase. The present study was carried out in order to elucidate as to how altered serotonin levels affect the day/night changes in the number of synaptic ribbon profiles. To this end, male Sprague-Dawley rats received a single dose of p-chlorophenylalanine (pCPA, 300 mg/kg body weight, 72 hours before sacrifice), which depresses tryptophan hydroxylase activity and hence pineal serotonin levels. Control animals received saline injections. Experimental and control animals were killed in the middle of the light period and in the middle of the dark period, respectively. The pineal glands were removed and bisected. One half was used for electron microscopic quantification of synaptic ribbon profile numbers, and the other half for determination of several biochemical parameters (see below). pCPA decreased pineal serotonin levels to c. 30% of the controls and synaptic ribbon profile numbers by c. 30%, both at day and night. The day/night rhythm of the two parameters did not appear to be affected. The rate-limiting enzyme of melatonin synthesis, serotonin N-acetyltransferase, which is only measurable at night, and pineal melatonin levels were not affected at night. The intermediary product of melatonin formation, N-acetylserotonin, was significantly depressed at night. It is concluded that serotonin plays a more important modulatory role on pineal synaptic ribbons than does melatonin.(ABSTRACT TRUNCATED AT 250 WORDS)