6533b854fe1ef96bd12aebaf
RESEARCH PRODUCT
Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance.
Ramon GonzalezJ BuesaS. FerrerDaniel Ramónsubject
ImmunologyGuinea PigsVirulenceMitosisCochliobolus heterostrophusmedicine.disease_causeMicrobiologyMicrobiologychemistry.chemical_compoundPlasmidTransformation GeneticTrichophytonmedicineAnimalsTrichophytonGenebiologyVirulenceDrug Resistance Microbialbiology.organism_classificationGrowth InhibitorsAnti-Bacterial AgentsTransformation (genetics)Blotting SouthernInfectious DiseaseschemistryDermatophyteParasitologyHygromycin BHygromycin BResearch Articledescription
A transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, Mol. Cell. Biol. 7:3297-3305, 1987). This plasmid confers hygromycin B resistance to T. mentagrophytes. The DNA was stably integrated into the fungal genome, and the number and sites of integrations varied among transformants. Transformant clones were capable of infecting guinea pigs. This system opens the way for the molecular genetic analysis of the interaction of T. mentagrophytes with epithelial animal tissues
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-09-01 | Infection and immunity |