Xenobiotic-metabolizing enzyme activities in hybrid cell lines established by fusion of primary rat liver parenchymal cells with hepatoma cells

6533b854fe1ef96bd12af60a

RESEARCH PRODUCT

Xenobiotic-metabolizing enzyme activities in hybrid cell lines established by fusion of primary rat liver parenchymal cells with hepatoma cells

D. Utesch E. Petzinger Michael Arand Franz Oesch Helmut Thomas

subject

Male 1303 Biochemistry Health Toxicology and Mutagenesis 10050 Institute of Pharmacology and Toxicology 610 Medicine & health Hybrid Cells Toxicology Biochemistry Cell Line Mixed Function Oxygenases Xenobiotics Cell Fusion Rats Sprague-Dawley chemistry.chemical_compound Liver Neoplasms Experimental 2307 Health Toxicology and Mutagenesis Tumor Cells Cultured Animals Enzyme inducer Epoxide hydrolase Pharmacology chemistry.chemical_classification biology digestive oral and skin physiology Cytochrome P450 3005 Toxicology General Medicine Glutathione Rats Enzyme 3004 Pharmacology chemistry Biochemistry Liver Cell culture Enzyme inhibitor biology.protein Microsome 570 Life sciences; biology

description

1. The activities of xenobiotic-metabolizing enzymes were determined in hybrid cell lines (hepatocytoma, HPCT) which have been established by fusion of liver parenchymal cells from adult rat (PC) with cells from a Reuber hepatoma cell line (FAO). 2. Cytochrome P450 was not measurable spectrophotometrically in FAO and HPCT. P450-dependent conversion of testosterone was below the detection limit in FAO and only marginally present in HPCT. 3. Microsomal and cytosolic epoxide hydrolase, glutathione S-transferase and phenol sulphotranserase were low or even below detection limit in FAO. These enzyme activities were significantly higher in HPCT and correspond to about 1-10% the activities measured in PC. 4. 1-Naphthol UPD-glucuronosyl transferase activity was about 20% in FAO and about 100% in HPCT compared to PC. 5. Metabolic conversion of benzo[a]pyrene was low in FAO, high in PC, and intermediate in HPCT. The presented data, however, do not allow the conclusion whether this intermediate rate is catalyzed by similar P450 isoenzymes as in PC. 6. Due to the easily measurable phase II-metabolizing enzyme activities HPCT may, however, be useful for in vitro enzyme induction or repression studies.

year	journal	country	edition	language
1992-12-01

10.5167/uzh-114016 https://doi.org/10.5167/uzh-114016