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RESEARCH PRODUCT
Calcium-binding sites in the inner ear after pure-tone stimulation
Ulf-rüdiger HeinrichW. J. MannJan Maurersubject
DiminutionBinding SitesTectorial membraneGuinea Pigschemistry.chemical_elementStimulationGeneral MedicineAnatomyCalciumBiologyGuinea pigBasilar membraneSoundmedicine.anatomical_structureAcoustic StimulationOtorhinolaryngologychemistryEar Innerotorhinolaryngologic diseasesmedicineAnimalsCalciumInner earsense organsBinding sitedescription
Five guinea pigs were exposed to an interrupted 90 dB SPL pure tone of 3.2 kHz for a total application time of 5 min. Following sound application all animals were decapitated and the cochleae were removed. After that, calcium-binding sites were located by the potassium pyroantimonate precipitation method. Another three animals served as control animals and did not receive the sound treatment. Findings confirmed previous studies showing the spatial arrangements of precipitate rich regions in the inner ear's two acellular structures (the basilar membrane and tectorial membrane) and the two cellular structures (the inner hair cells and Huschke's teeth). By using semiquantitative evaluation we found a relative diminution of precipitable calcium in inner hair cells and in Huschke's teeth of sound-exposed animals when compared to untreated animals.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-05-01 | European Archives of Oto-Rhino-Laryngology |