Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients.

6533b855fe1ef96bd12b0048

RESEARCH PRODUCT

Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients.

Fabienne Bon Pierre Pothier Pierre Lebon Jérôme Kaplon D. Gendrel Mathie Lorrot H. Giraudon M. Wolfer Katia Ambert-balay

subject

Aichi virus Epidemiology MESH : Prevalence MESH : Diarrhea MESH : Kobuvirus Disease Outbreaks Feces MESH : Child MESH: Picornaviridae Infections MESH: Child MESH: Animals MESH: Genetic Variation MESH: Phylogeny Child Phylogeny 0303 health sciences Cross Infection MESH: Kobuvirus MESH : Reverse Transcriptase Polymerase Chain Reaction MESH: Feces virus diseases MESH : Infant MESH: Infant 3. Good health MESH : Gastroenteritis MESH: Diarrhea MESH: Seafood [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Child Preschool [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Aichi virus MESH : Cross Infection Microbiology (medical)Diarrhea MESH : Community-Acquired Infections Kobuvirus Molecular Sequence Data [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Ostreidae 03 medical and health sciences MESH : Adolescent Humans MESH : Disease Outbreaks MESH: Prevalence MESH: Adolescent MESH : Seafood MESH: Humans MESH: Molecular Sequence Data Picornaviridae Infections 030306 microbiology MESH: Child Preschool MESH : Humans Outbreak Genetic Variation Infant DNA Virology MESH: Gastroenteritis Seafood MESH : Sequence Analysis DNA MESH: Sequence Analysis DNA MESH : Molecular Sequence Data MESH : Child Preschool [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology MESH: Reverse Transcriptase Polymerase Chain Reaction Genotype Prevalence MESH: Disease Outbreaks [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH : Picornaviridae Infections biology Reverse Transcriptase Polymerase Chain Reaction Incidence (epidemiology)MESH: Infant Newborn Gastroenteritis Community-Acquired Infections Diarrhea MESH: Community-Acquired Infections Kobuvirus France medicine.symptom Sequence Analysis Adolescent MESH : Infant Newborn MESH : Genetic Variation Genetic variation medicine Animals Preschool MESH : France Feces 030304 developmental biology MESH : Ostreidae Infant Newborn MESH: Cross Infection MESH : Phylogeny Sequence Analysis DNA MESH : Feces biology.organism_classification Newborn Ostreidae MESH: France MESH : Animals

description

ABSTRACT Aichi virus has been proposed as a causative agent of gastroenteritis. A total of 457 stool specimens from children hospitalized with acute diarrhea and 566 stool specimens from adults and children involved in 110 gastroenteritis outbreaks were screened for the presence of Aichi virus by reverse transcription-PCR (RT-PCR) amplification of the genomic region of the 3C and 3D (3CD) nonstructural proteins. Our results show a low incidence of Aichi virus in pediatric samples and the existence of mixed infections with other microbiological agents in some cases. From the outbreak survey, it appears that the presence of Aichi virus is an indicator of mixed infections causing gastroenteritis outbreaks and that it could be involved in half of the oyster-associated outbreaks. A second RT-PCR was developed to amplify a part of the VP1 gene. The phylogenetic analysis showed a good correlation between the two classifications based on 3CD and VP1 gene sequences and revealed the prevalence of genotype A in France. It also allowed us to partially describe an Aichi virus strain that could represent a new genotype, thus suggesting the existence of a certain diversity.

year	journal	country	edition	language
2008-04-01

https://hal.archives-ouvertes.fr/hal-00452284/file/ambert_2008.pdf