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RESEARCH PRODUCT

Tentative immunohistochemical demonstration of melatonin in the rat pineal gland

Josephine ArendtLutz VollrathDagmar Freund

subject

Maleendocrine systemmedicine.medical_specialtyPathologyHistologyFluorescent Antibody TechniquePineal ParenchymaBiologyPineal GlandPathology and Forensic MedicinePinealocyteMelatoninRat Pineal GlandPineal glandInternal medicineParenchymamedicineAnimalsMelatoninCell BiologyMarginal zoneCircadian RhythmRatsEndocrinologymedicine.anatomical_structureImmunohistochemistryRabbitshormones hormone substitutes and hormone antagonistsmedicine.drug

description

In the present study an attempt was made to demonstrate melatonin in the rat pineal gland by means of immunohistochemistry. The anti-body used was raised against 5-methoxy-N-acetyltryptophan which is chemically similar to melatonin. Specific fluorescence was demonstrable only in pineals from rats killed during the night, when melatonin formation is high. It was restricted to parenchymal cells lying in a marginal zone of the organ. These results are discussed in relation to a subdivision of the pineal parenchyma into cortical and medullary areas.

yearjournalcountryeditionlanguage
1977-07-01Cell and Tissue Research
https://doi.org/10.1007/bf00219983