6533b855fe1ef96bd12b0946
RESEARCH PRODUCT
Identification of the Yersinia enterocolitica urease beta subunit as a target antigen for human synovial T lymphocytes in reactive arthritis.
K H Meyer Zum BüschenfeldeB. FleischerElisabeth HermannPeter Probstsubject
musculoskeletal diseasesProtein subunitT-LymphocytesImmunologyMolecular Sequence DataBiologyLymphocyte ActivationMicrobiologyEpitopeMicrobiologyAntigenProhibitinsSynovial FluidSynovial fluidHumansAmino Acid SequenceYersinia enterocoliticaHLA-DR AntigenYersinia enterocoliticaAntigens BacterialSequence Homology Amino AcidArthritisT lymphocyteHLA-DR Antigensbiology.organism_classificationbacterial infections and mycosesUreaseInfectious DiseasesParasitologyBacterial antigenResearch Articledescription
The local T-cell response to bacterial antigens is involved in the pathogenesis of reactive arthritis (ReA). Here, we have identified a 19-kDa antigen of Yersinia enterocolitica O:9 recognized by Yersinia-specific synovial fluid CD4+ T cells in two patients with Yersinia-induced ReA. N-terminal amino acid sequencing of this protein revealed that it was identical to the 19-kDa urease beta subunit of Y. enterocolitica O:9. This protein has previously been shown to be arthritogenic in preimmunized rats after intra-articular injection. Analysis of the T-cell response to this protein showed that it contains several T-cell epitopes, one of which cross-reacts with other enterobacteria not able to induce ReA. This indicates that the arthritogenicity of the 19-kDa antigen is not a property of the 19-kDa protein alone but is dependent on its expression in bacteria able to induce ReA.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-10-01 |