6533b855fe1ef96bd12b0b6b

RESEARCH PRODUCT

Chromatogrphic Profiles of Polyphenols and Terpenoids in the Herbs of Some Thymus L. Represntatives

subject

description

The aim of the work. To perform the identification of phenolic compounds and terpenoids in the aerial parts of Thymus pulegioides L. (cultivar ‘2/6-07’), Thymus richardii Pers. (cultivar 'Fantasy') and Thymus vulgaris L. (cultivar ‘Jalos’) using thin layer chromatography (TLC), as well as to conduct the gaschromatographic analysis of Thymus richardii essential oil. Materials and Methods. The TLC method was used in this study for the qualitative analyses of polyphenols and non-polar compounds in the aerial part of three Thyme (Thymus L.) species from the Lamiaceae Martinov family cultivated in Kherson region (Ukraine). The volatile compounds were determined in the essential oil of Thymus richardii by gas chromatography mass spectrometry (GC/MS) method. Results and Discussion. Rosmarinic and caffeic acids were identified in the methanol extracts of all three Thymus representatives. Luteolin was revealed distinctly only in the Thymus richardii herb whilst the weak zone of apigenin was found only in Thymus vulgaris. The TLC-analysis of terpenoids showed the presence of the clear spot of thymol only in the methylene chloride extract of Thymus vulgaris aerial part. The weak zones of β-sitosterol were visible in the chromatograms of all three Thymus species. The major volatile constituent of the Thymus richardii essential oil was linalool (38.60 %), followed by linalyl acetate (18.26 %) and thymol (8.41 %). These predominant compounds possess significant therapeutic potential. Conclusions. The used chromatographic techniques could be regarded as simple and reliable methods for the phytochemical authentication of the studied Thymus cultivars.