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RESEARCH PRODUCT
NG2-Glia Transiently Overcome Their Homeostatic Network and Contribute to Wound Closure After Brain Injury
Axel Von StreitbergSarah JäkelJaime Eugenin Von BernhardiJaime Eugenin Von BernhardiChristoph StraubeFelix BuggenthinCarsten MarrLeda DimouLeda Dimousubject
polarizationnervous systemQH301-705.5proliferationstab wound injuryin vivo two-photon imagingBiology (General)migrationdescription
In the adult brain, NG2-glia represent a cell population that responds to injury. To further investigate if, how and why NG2-glia are recruited to the injury site, we analyzed in detail the long-term reaction of NG2-glia after a lesion by time-lapse two-photon in vivo microscopy. Live imaging over several weeks of GFP-labeled NG2-glia in the stab wounded cerebral cortex revealed their fast and heterogeneous reaction, including proliferation, migration, polarization, hypertrophy, or a mixed response, while a small subset of cells remained unresponsive. At the peak of the reaction, 2–4 days after the injury, NG2-glia accumulated around and within the lesion core, overcoming the homeostatic control of their density, which normalized back to physiological conditions only 4 weeks after the insult. Genetic ablation of proliferating NG2-glia demonstrated that this accumulation contributed beneficially to wound closure. Thus, NG2-glia show a fast response to traumatic brain injury (TBI) and participate in tissue repair.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-04-01 | Frontiers in Cell and Developmental Biology |