6533b855fe1ef96bd12b1316
RESEARCH PRODUCT
Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor.
Enrico GrattonLeonel MalacridaLeonel MalacridaEstella RaoEstella Raosubject
0301 basic medicineDigital image correlationIntravital MicroscopyImage ProcessingGreen Fluorescent ProteinsClinical SciencesChemicalCHO CellsGeneral Biochemistry Genetics and Molecular BiologyDiffusion AnisotropyArticleFluorescenceDiffusion03 medical and health sciencesConnectivity mapsCricetulusComputer-AssistedModelsMolecular motionImage Processing Computer-AssistedAnimalsEpidermal growth factor receptorDiffusion (business)Diffusion anisotropyMolecular BiologyImage resolutionPhysicsMicroscopyFluorescence fluctuation spectroscopybiologyMethod of analysisErbB Receptors030104 developmental biologyMicroscopy FluorescenceModels ChemicalBarrier to diffusionbiology.proteinBiological systemAlgorithmsdescription
Image correlation analysis has evolved to become a valuable method of analysis of the diffusional motion of molecules in every points of a live cell. Here we compare the iMSD and the 2D-pCF approaches that provide complementary information. The iMSD method provides the law of diffusion and it requires spatial averaging over a small region of the cell. The 2D-pCF does not require spatial averaging and it gives information about obstacles for diffusion at pixel resolution. We show the analysis of the same set of data by the two methods to emphasize that both methods could be needed to have a comprehensive understanding of the molecular diffusional flow in a live cell.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-05-01 |