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RESEARCH PRODUCT
Deoxyribonucleases in Herpes simplex Virus Type 1 and 2 Infected Primary Rabbit Kidney Cells
Rudolf K. ZahnFalke DZöllner Ejsubject
chemistry.chemical_classificationDeoxyribonucleasesbiologyChemistryDeoxyribonucleaseCell Transformation ViralKidneymedicine.disease_causeVirologyGeneral Biochemistry Genetics and Molecular BiologyEnzyme assayKineticsHerpes simplex virusEnzymemedicinebiology.proteinRabbit kidneyAnimalsSimplexvirusRabbitsDeoxyribonucleasesCells Cultureddescription
Abstract In primary rabbit kidney cells infected with herpes simplex virus four different neutral deoxyribonuclease activities can be detected by means of the deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by discelectrophoresis. The method is suitable to follow independently the change in each activity of the different enzymes using only about 5 × 105 cells for each assay during the time-course of infection. Under these conditions one enzyme activity is constant, two disappear while the activity of a fourth one present only in infected cells, increases.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1980-09-01 | Zeitschrift für Naturforschung C |