6533b855fe1ef96bd12b14f3

RESEARCH PRODUCT

Molecular Characterization of the Leucine Plasmid from Buchnera aphidicola , Primary Endosymbiont of the Aphid Acyrthosiphon pisum

Beatriz SabaterTeresa SolerAmparo LatorreFrancisco J. Silva

subject

OperonInverted repeatMolecular Sequence DataMinisatellite RepeatsBiologyOrigin of replicationApplied Microbiology and BiotechnologyMicrobiologyOpen Reading FramesPlasmidBuchneraLeucinePhylogeneticsAnimalsSymbiosisPhylogenyGeneticsBase Sequencefood and beveragesGeneral Medicinebiochemical phenomena metabolism and nutritionbiology.organism_classificationAcyrthosiphon pisumTerminator (genetics)Genes BacterialAphidsBuchneraMicrosatellite RepeatsPlasmids

description

The complete sequence of the leucine plasmid of Buchnera aphidicola from the aphid Acyrthosiphon pisum (pLeu-BAp) is reported. Its gene organization was concordant with those of other leucine plasmids of Buchnera from aphids of the Aphidini and Macrosiphini tribes. Three inverted repeats are present in pLeu-BAp. Two of them are also present in pLeu from the family Aphididae: (i) SIR1, located downstream the leucine operon, resembles a rho-independent terminator of transcription, and (ii) LIR1, located upstream of the leucine operon, is suggested to be involved in transcription termination or messenger stability. The third, located near the putative ATGC repeats involved in the origin of replication, is specific in aphids of the Macrosiphini tribe. Phylogenetic analyses based on sequences of leuA, leuB, leuC, leuD, repA1 and ORF1 showed a closer relationship between Buchnera (A. pisum) and Buchnera (Diuraphis noxia). However, tree topologies indicate that the split between both aphid species took place soon after the formation of the Macrosiphini lineage.

yearjournalcountryeditionlanguage
2000-02-25Current Microbiology
https://doi.org/10.1007/s002849910052