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RESEARCH PRODUCT
Spectrofluorimetric Determination of Iproniazid and Isoniazid in a FIA System Provided with a Solid-Phase Reactor
J. Martínez CalatayudJ. A. García BautistaJ. V. García Mateosubject
Detection limitChromatographyChemistryBiochemistry (medical)Clinical BiochemistryIsoniazidFluorescence spectrometryBiochemistryDosage formAnalytical ChemistryIproniazidReagentPhase (matter)ElectrochemistrymedicineQuantitative analysis (chemistry)Spectroscopymedicine.drugdescription
Abstract A FIA assembly is proposed for the fluorimetric determination of iproniazid and isoniazid. The oxidation of both drugs is carried out by H2O2. The excess of reagent is destroyed and removed from the flow-injection manifold by means of a metallic copper reactor which acts as catalyst in the decomposition of H2O2 and a home made debubbler. The calibration graphs were linear up to 14 μg ml−1 and 10 μg ml−1 (n=6) for iproniazid and isoniazid, respectively, with limits of detection of 0.008 μg ml−1 and 0.005 μg ml−1. The sample throughput was 24 h−1 for both drugs. The influence of foreign compounds was studied and the procedure was applied to determination of iproniazid and isoniazid in pharmaceutical formulations.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-05-01 | Analytical Letters |