6533b856fe1ef96bd12b1c8f
RESEARCH PRODUCT
Dimethylsulfoxide as carrier in enzyme cytochemistry.
Jürgen Reisssubject
CercosporellaHistologyintegumentary systembiologyHyphaStaining and LabelingHistocytochemistryorganic chemicalsSuccinate dehydrogenasefungiCell BiologyStainingIncubation periodMedical Laboratory TechnologyBiochemistryMucilagebiology.proteinCytochemistryDimethyl SulfoxideMitosporic FungiMolecular BiologyIncubationdescription
Addition of dimethylsulfoxide (DMSO) to the incubation medium of succinate dehydrogenase in a concentration of 10% enhances the staining reaction in the hyphae of the fungus Cercosporella herpotrichoides after an incubation period of 15 min. Controls without DMSO remain unstained. DMSO causes a rapid penetration of the components of the medium through the mucilage that covers the hyphae.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1971-01-01 | Histochemie. Histochemistry. Histochimie |