6533b856fe1ef96bd12b1e50
RESEARCH PRODUCT
Analysis of Drosophila salivary gland, epidermis and CNS development suggests an additional function of brinker in anterior-posterior cell fate specification.
Lisa MeadowsUwe LammelHarald Saumwebersubject
Central Nervous SystemEmbryologyReceptors SteroidEmbryo NonmammalianMutantLocus (genetics)OrganogenesisBiologyCell fate determinationSalivary GlandsNeuroblastBacterial ProteinsmedicineAnimalsDrosophila ProteinsAdhesins BacterialGeneBody PatterningEmbryonic InductionHomeodomain ProteinsSalivary glandGenetic Complementation TestNeuropeptidesChromosome MappingGene Expression Regulation DevelopmentalCell DifferentiationAnatomyPhenotypeCell biologyRepressor Proteinsmedicine.anatomical_structureEpidermal CellsMutationInsect ProteinsDrosophilaEpidermisDevelopmental BiologyTranscription Factorsdescription
Salivary glands are simple structured organs which can serve as a model system in the study of organogenesis. Following a large EMS mutagenesis we have identified a number of genes required for normal salivary gland development. Mutations in the locus small salivary glands-1 (ssg-1) lead to a drastic reduction in the size of the salivary glands. The gene ssg-1 was cloned and subsequent sequence and genetic analysis showed identity to the recently published gene brinker. The salivary gland placode in brinker mutants appears reduced along both the anterior-posterior and dorso-ventral axis. Analysis of the brinker cuticle phenotype revealed a similar loss of anterior-posterior as well as lateral cell fates. The abdominal ventral denticle belts show a reduced number of setae in the first denticle row. Furthermore, we observed a preferential loss of lateral neuroblasts in the anterior parasegment. Together, these phenotypes suggest that brinker not only plays a role in dorso-ventral but also in anterior-posterior axis patterning.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-04-01 | Mechanisms of development |