Mammalian glial cells in culture synthesize acetylcholine.

6533b856fe1ef96bd12b1e5f

RESEARCH PRODUCT

Mammalian glial cells in culture synthesize acetylcholine.

Torsten Reinheimer Kurt Racké Holger Klapproth Franz-josef Schneider Ignaz Wessler Rudolf Hammer

subject

Choline O-Acetyltransferase chemistry.chemical_compound Mice medicine Animals Neurotransmitter Cells Cultured Chromatography High Pressure Liquid Pharmacology Acetylcholine Bromide Microglia biology General Medicine Rat brain Choline acetyltransferase Enzyme assay Acetylcholine Cell biology Rats medicine.anatomical_structure chemistry Biochemistry Animals Newborn Astrocytes biology.protein Microglia Acetylcholine Astrocyte medicine.drug

description

In the present study we demonstrate that acetylcholine is synthesized by cultured mammalian glial cells identified by cell-type specific markers. Primary cultures of rat brain astrocytes or microglia contained 2.0 and 1.6 pmol acetylcholine/10(6) cells on average respectively. Astrocyte cultures established from neonatal mouse brain contained even more acetylcholine (about 80 pmol acetylcholine/10(6) cells). Primary cultures of rat brain astrocytes showed choline acetyltransferase (ChAT) enzyme activity of 3 nmol/mg protein/h; ChAT activity was blocked by 10 microM bromoacetylcholine. In conclusion, these data demonstrate the synthesis of the "neurotransmitter" acetylcholine in cultured glial cells, a finding which opens a new view upon the role of acetylcholine in mammalian brain.

year	journal	country	edition	language
1997-10-28	Naunyn-Schmiedeberg's archives of pharmacology

10.1007/pl00005107 https://pubmed.ncbi.nlm.nih.gov/9402051

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