6533b856fe1ef96bd12b282d

RESEARCH PRODUCT

Analysis of synaptic bodies in the Sprague–Dawley rat pineal gland under extreme photoperiods

subject

description

Synaptic bodies (SBs) are small, prominent organelles in pinealocytes, most probably involved in signal transduction processes. To check the influence of the photoperiod on their shape plasticity and number we chose two extreme lighting conditions, i.e. 20 h of illumination followed by 4 h of darkness (LD 20:4) versus (LD 4:20). Pineal glands were assessed at 0, 4 and 13 h after dark onset. Under both conditions reconstructed SBs were plates or ribbons but never spheres and there were no obvious differences in morphology. Photoperiodic changes in SB profile size and number were investigated: application of the established method for SB quantification based on single section profile counts (SSPC) of areas showed a significant increase of SB profiles under LD 20:4. However, it has to be noted that SSPC depend on both, number and size of the structures. In contrast to this, modification of the disector counting method, also applied for unbiased quantification of whole SBs, revealed that rat pinealocytes show insignificantly more SBs under LD 20:4 than under 4:20 conditions. The lengths of the SB profiles, which were first measured under different conditions in this study, depend on SB size. They increased significantly under LD 20:4. In conclusion, we detected only an increase in SB size but not in their number. We further prove that, at least for SBs, it is of no value to calculate disector levels from SSPCs.