6533b856fe1ef96bd12b2836
RESEARCH PRODUCT
Relief of microRNA-Mediated Translational Repression in Human Cells Subjected to Stress
Suvendra N. BhattacharyyaRegula HabermacherEllen I. ClossUrsula MartinéWitold Filipowiczsubject
AU-rich elementUntranslated regionBiochemistry Genetics and Molecular Biology(all)Three prime untranslated regionPolysomeP-bodiesELAV-Like Protein 1BiologyMolecular biologyPsychological repressionGeneral Biochemistry Genetics and Molecular BiologyDerepressiondescription
SummaryIn metazoans, most microRNAs imperfectly base-pair with the 3′ untranslated region (3′UTR) of target mRNAs and prevent protein accumulation by either repressing translation or inducing mRNA degradation. Examples of specific mRNAs undergoing microRNA-mediated repression are numerous, but whether the repression is a reversible process remains largely unknown. Here we show that cationic amino acid transporter 1 (CAT-1) mRNA and reporters bearing its 3′UTR can be relieved from the microRNA miR-122-induced inhibition in human hepatocarcinoma cells subjected to different stress conditions. The derepression of CAT-1 mRNA is accompanied by its release from cytoplasmic processing bodies and its recruitment to polysomes. The derepression requires binding of HuR, an AU-rich-element binding protein, to the 3′UTR of CAT-1 mRNA. We propose that proteins interacting with the 3′UTR will generally act as modifiers altering the potential of miRNAs to repress gene expression.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-01 | Cell |