FRET sistēmas aprobācija CRAC jonu kanālu pētījumiem

6533b856fe1ef96bd12b2ad6

RESEARCH PRODUCT

FRET sistēmas aprobācija CRAC jonu kanālu pētījumiem

subject

HEK 293 proteīnu mijiedarbība FRET Bioloģija CRAC YFP

description

Fluorescentās rezonanses enerģijas pārneses metodei ir plašs pielietojums mūsdienu zinātnē proteīnu mijiedarbības un konformācijas maiņas petījumos. Tā kā Latvijā pētniecības metode nav izmantota, nepieciešama pilnīga instrumentu, ietekmējošo faktoru un parametru apguve no pašiem pamatiem. Bakalaura darbā “FRET sistēmas aprobācija CRAC jonu kanālu pētījumiem” veikti trīs galvenie eksperimenti, izmantotojot STIM1 ar CFP fluorescento proteīnu C vai N galā molekulas mijiedarbība ar ORAI1, kam N galā YFP proteīns, kuru gaitā uzlabota šūnu audzēšanas tehnika, FRET mikroskopa konfigurācija, kvalitatīvu attēlu uzņemšana un kvalitatīva datu apstrāde. Iegūtas tehniskas prasmes un zināšanas augstas precizitātes FRET datu ieguvei.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/33183

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