Author: Laura Hippe

0000000000999628

AUTHOR

Laura Hippe

showing 2 related works from this author

FRET fluorescento proteīnu balstītu pH sensoru izveide

2018

Iekššūnas vides pH ir būtisks parametrs, kas raksturo šūnā notiekošos procesus, kā arī šūnas vitalitāti. Fluorescento proteīnu pielietošana pH noteikšanai ir daudzsološa metode. Pieejami atšķirīgas pH jutības fluorescentie proteīni, tie neuzrāda augstu toksicitāti un invazivitāti, padarot tos piemērotus dzīvu šūnu pētījumiem. Maģistra darba mērķis bija izveidot fluorescento proteīnu saturošus konstruktus ar atšķirīgu pH jutības slieksni, izmantojot Aquamarine, ECFP, EYFP un Citrine fluorescentos proteīnus, kas darbosies kā pH sensoru sistēma dzīvās šūnās, izmantojot FRET metodi. Trīs konstrukciju izveide (pAqua-HL3-Citrine, pAqua-HL3-EYFP, pECFP-HL3-EYFP) veikta ar dažādām metodiskajām piee…

pH sensorsFRETklonēšanaBioloģijafluorescents proteīns

researchProduct

FRET sistēmas aprobācija CRAC jonu kanālu pētījumiem

2016

Fluorescentās rezonanses enerģijas pārneses metodei ir plašs pielietojums mūsdienu zinātnē proteīnu mijiedarbības un konformācijas maiņas petījumos. Tā kā Latvijā pētniecības metode nav izmantota, nepieciešama pilnīga instrumentu, ietekmējošo faktoru un parametru apguve no pašiem pamatiem. Bakalaura darbā “FRET sistēmas aprobācija CRAC jonu kanālu pētījumiem” veikti trīs galvenie eksperimenti, izmantotojot STIM1 ar CFP fluorescento proteīnu C vai N galā molekulas mijiedarbība ar ORAI1, kam N galā YFP proteīns, kuru gaitā uzlabota šūnu audzēšanas tehnika, FRET mikroskopa konfigurācija, kvalitatīvu attēlu uzņemšana un kvalitatīva datu apstrāde. Iegūtas tehniskas prasmes un zināšanas augstas p…

HEK 293proteīnu mijiedarbībaFRETBioloģijaCRACYFP

researchProduct

PEPhysical Sciences and Engineering
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LSLife Sciences
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