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RESEARCH PRODUCT
Detection of antigen-specific T-cells with MHC/peptide-tetramer-complexes
Glenn TullyMarkus MaeurerElke Jägersubject
Autoimmune diseaseMelanomaImmunologyBiologymedicine.diseaseMajor histocompatibility complexMicrobiologyIn vitroCTL*Infectious DiseasesImmune systemTetramerImmunologymedicinebiology.proteinImmunology and AllergyEx vivodescription
Abstract Tetramer analysis is a novel technique in immunological research that has dramatically changed our knowledge of the immune response to viral and bacterial pathogens, to tumors and in autoimmune disease. Through the formation of major histocompatibility complex (MHC)/peptide-tetrameric complexes it can provide accurate counts of antigen-specific T-cells and it allows their phenotypical and functional analysis. We review the strengths of this method and use the analysis of peripheral blood lymphocytes (PBL) from a patient with melanoma as a paradigm for the detection of tetramer-binding T-cells. The implementation of tetramer-guided analysis in immunomonitoring allows the ex vivo testing of antigen-specific T-cells without the need of in vitro manipulation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-10-01 | Clinical and Applied Immunology Reviews |