6533b856fe1ef96bd12b2fe0

RESEARCH PRODUCT

Activation of Mast Cells by Streptolysin O and Lipopolysaccharide

Iwan WalevAngela ValevaMichael StassenEdgar Schmitt

subject

Reporter genegenetic structuresElectroporationDegranulationTransfectionMast cellCell biologychemistry.chemical_compoundmedicine.anatomical_structurechemistryIonomycinmedicineTumor necrosis factor alphaStreptolysin

description

This chapter provides protocols to measure the reversible permeabilization of mast cells by streptolysin O (SLO) and to follow SLO-induced activation of mast cells by monitoring degranulation, activation of mitogen-activated protein kinases, and production of tumor necrosis factor-alpha. A method that uses SLO to deliver molecules into the cytosol of living cells also is described. Furthermore, we outline a procedure to measure the activation of nuclear factor-kappaB by lipopolysaccharide and ionomycin using transfection of mast cells with reporter genes by electroporation. These protocols should be widely applicable in mast cell research.

yearjournalcountryeditionlanguage
2005-08-18
https://doi.org/10.1385/1-59259-967-2:393